Cleavable C-terminal His-tag

c.collyer at c.collyer at
Fri Jan 24 00:19:16 EST 1997

> I want to know if there is an appropriate restriction site that can be 
> inserted between the end of my protein and the his-tag?
> I want to use a common cleavage site such as thrombin, but I need to be able
> to remove all (or most) the amino acid residues that form the thrombin 
> recognition site.
> Does someone have any suggestions?
> Is there any published material on this area?

>Thanks in advance

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