jkropp at itsa.ucsf.edu
Thu Jan 23 15:55:09 EST 1997
In article <jsg-2101971645050001 at jsgpb1.path.cam.ac.uk>,
jsg at mole.bio.cam.ac.uk (James Good) wrote:
> Concentrations in what range? Has anyone used these reagents in GC-rich
> PCR with any success? I work on a bacterium whose genome is 67% GC, making
> PCR somewhat trying!
See Chavet,E., et.al.,1995, NAR, 23:16, 3343-3344.
I had a 70% G/C template that was totally refractory until I juiced it
with 50mM TMAC *and* 10% DMSO.
Hope it helps,
"no brains...no headache!"
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