Degraded DNA Cleanup?

Dr. Duncan Clark duncan at
Thu Jan 23 04:12:52 EST 1997

In article <5c01fq$jfa at>, Dima Klenchin <klenchin at facs> writes
>In article <32E397D7.60C8 at GENVEC.COM>, CANTOLUPO at GENVEC.COM (Lou Cantolupo) 
>->Anyone have a good protocol to clean up degraded DNA?  My fragment is
>->about 35Kb (whole adenovirus DNA), VERY PRECIOUS, and is marred by a lot
>->of degraded DNA.  I'd like to get rid of all the junk.  I've
>->reprecipitated and that didn't help much.  Any suggestions?  Thanx in
>I would try cesium gradient. 

I doubt AD2 will separate from degraded DNA on CsCl as they will both be
linear. If your degraded fragments are smaller then I would gel purify
your large fragment. If you have a lot of sample, sucrose gradient
should size select OK. If you only have trace amounts then maybe long
PCR of the whole lot or spli it into 17.5kb or 12kb chunks. 

The problem with being on the cutting edge is that you occasionally get 
sliced from time to time....

Dr. Duncan Clark
DNAmp Ltd.
TEl/FAX 01252376288

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