3->5 rate of Klenow
ADVANCED TECH (CAMB)
atc.biotech at dial.pipex.com
Thu Jan 23 16:13:03 EST 1997
Does anybody know what the rate of the 3' to 5' exonuclease activity of the Klenow
fragment is (in terms of bases/sec). I intend to use it to end label a 100 bp probe.
My protocol says incubate for 10min at R.T. before the polymerase reaction but I think
this may be too long and will completely digest my probe. Does anyone have a better
protocol, mine is not very detailed.
kin (kin.cheung at virgin.net)
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