Protein G sepharose vs prot A with bridge

Mike Holloway holloway at
Thu Jan 23 13:07:16 EST 1997

Does anyone out there have experience enough to suggest which is better?  I
want to immunoprecipitate with a monoclonal antibody.  It looks as though
protean G sepharose should be the choice for substrate, but I note that most
protocols I've seen use a rabbit anti-mouse bridge and protean A sepharose.
Is this better than prot G?

Mike Holloway
holloway at

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