cloning of large genomic DNA fragment

James Smith jsmith at bcm.tmc.edu
Fri Jan 24 11:10:43 EST 1997


Hi, everyone, 

I'm trying to clone large piece of genomic DNA (about 10-15 kb) into
some types of vectors for sequencing. I'm having some problem in cloning
these fragments into PBluescript. Are there any other vectors can be
used in efficient cloning of large DNA fragments or are there some
particular cautions to take during cloning process? If possible, please
email your response to "xswanson at bcm.tmc.edu". Your help will be really
appreciated!!!

Xin Hang
Baylor College of medicine, Houston



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