Dissolving plasmid DNA afterprecipitation
toth at CRIC.COM
Fri Jan 24 12:12:25 EST 1997
What is your template isolation method. If it is Quiagen or
alike with a solid support column, I have seen where some of this
support can come through into samples. These then pellet down with
ppt DNA and will not resuspend when dissolving your plasmid. If you
are using these methods of template prep then try a control of just
H2O over the column and see if you get solid pellet/ppt. Hope this
helps, good luck.
Genome Therapeutics Corp.
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Waltham, MA 02154
tel: 617-893-5007 X2551
617-398-2551 (Direct Dial)
toth at cric.com
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