What is the SMALLEST DNA BINDING DOMAIN?

Neil Clarke Neil.Clarke at qmail.bs.jhu.edu
Fri Jan 17 09:36:58 EST 1997


In article <E406Iu.C9C at gpu.utcc.utoronto.ca>, lamoran at gpu.utcc.utoronto.ca
(L.A. Moran) wrote:


> This request brings up another issue that might be worth discussing. Should
> these DNA binding regions be referred to as "domains" or "motifs"? 
> .....................
> If it's really a
> distinct "domain" then most of those who responded have not been helpful
> since they have concentrated on "motifs".

Actually, the homeodomain is a real domain by any criterion you choose. 
Although only sixty amino acids, fold and unfold reversibly, have
well-defined tertiary structures with Tm values above physiological
temperatures, etc.  (In fact, we've shown that a 51 amino fragment of the
homeodomain is structurally very similar to the full-length homeodomain,
though it can't bind DNA because its missing the N-terminal DNA binding
tail)

-- 
Neil Clarke
neil.clarke at qmail.bs.jhu.edu



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