E.coli for Electroporation

eric anderson e-anderson at ski.mskcc.org
Sun Jan 26 13:50:25 EST 1997


In article <Pine.OSF.3.95.970125173430.2874A-100000 at saturn.mahidol.ac.th>,
Panapat Uawithya <u3601087 at mahidol.ac.th> wrote:

> Hello  all,
>  We are now using E.Coli JM109 for transformation by CaCl2. But it didn't
> produce good enough efficiency. Can it be use for electroporation or not?
> If it can't , can you suggest any E.coli strain that similar to this
> strain but can be use for electroporation.
>                                         Thank you in advance,
>                                         Panapat Uawithya
> 
in my experience, the JM strains produce miserable results with
electroporation.  try using DH5's.  

eric

-- 
Eric C. Anderson
Memorial Sloan-Kettering Cancer Center
Sloan-Kettering Institute
1275 York Ave. Box 470
New York, NY  10021
(212) 639-2977
e-anderson at ski.mskcc.org



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