Quntifying small amounts of DNA reliably

s.pritchard mmd280 at sysc.abdn.ac.uk
Mon Jan 27 08:52:49 EST 1997


Hi

I am working on LOH in lung cancer and have to use paraffin embedded tissue
as the source material.The DNA is recovered from the tissue after standard
Haemotoxylin/eosin staining .PCR is performed on this material with no further
purification so as to minimise further degradation.The yields are obviously
small and therefore I can not estimate the conc. by running it on a gel and 
i presume that such crudely prepared DNA would be useless for analysis on
a spec. Any ideas?

Thanks in advance

Stuart Pritchard

mmd280 at abdn.ac.uk



More information about the Methods mailing list