Quntifying small amounts of DNA reliably

Francois Coulier coulier at infobiogen.fr
Mon Jan 27 15:33:46 EST 1997


On 27 Jan 1997, s.pritchard wrote:

> Hi
> 
> I am working on LOH in lung cancer and have to use paraffin embedded tissue
> as the source material.The DNA is recovered from the tissue after standard
> Haemotoxylin/eosin staining .PCR is performed on this material with no further
> purification so as to minimise further degradation.The yields are obviously
> small and therefore I can not estimate the conc. by running it on a gel and 
> i presume that such crudely prepared DNA would be useless for analysis on
> a spec. Any ideas?
> 
> Thanks in advance
> 
> Stuart Pritchard
> 
> mmd280 at abdn.ac.uk
> 
> 
You can spot 1 microliter of your DNA on an Et. Br. agarose plate, and
compare the fluoresecence intensity to known standard, spotted on the same
plate. It is quite sensitive, the accuracy depending mostly on your eyes!
Hope this help.


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Francois Coulier
INSERM Unite 119
27 bd Lei Roure
13009 Marseille
France

tel 33 (0) 4 91 75 84 11
fax 33 (0) 4 91 26 03 64)
email: coulier at infobiogen.fr
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