TBE vs. TAE

Przemko Tylzanowski przemko at sgi.celgen.kuleuven.ac.be
Mon Jan 27 10:50:15 EST 1997


Tom Duncan wrote:
> 
> John Ladasky wrote:
>> For several years now, our lab has used TTE buffer (TRIS-Taurine-EDTA)
> instead of TBE buffer for both agarose gels and sequencing gels. For agarose
> gels, it has as good a buffering capacity as TBE, as good or better
> resolution of DNA bands (0.2-10 kb). TTE buffer does not have the
> precipitation problems seen with TBE, and I have used year-old 20X stocks
snip

I use TBE (0.5x) for eveything and have no problms. I like the
resolution and, especially, the speed (TAE heats up easily). However i
would be interested in the taurine. Could you give me more details
(conc. and source)?

Przemko



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