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[Julie Roberts]

Yang Y.L. wrote:
> 
> Dear netter:
> I have problems in my probe design in Northern blot experiments
> PLEASE give me some suggestion !!
> 
> How Long dose PROBE work at its maximal efficiency ??
> Well 123 base too SHORT ??
> 
> thanks


I have used oligo probes as short as 30 bases with good success. A 123
base probe should be fine. I'm using ExpressHyb from Clonetech.
Wonderful stuff, we get beautiful blots very fast.

Julie
jroberts at rascal.med.harvard.edu