Efficient method for analysis of recombinants?

Dr. Duncan Clark duncan at genesys.demon.co.uk
Tue Jan 28 11:00:04 EST 1997


In article <5cge10$bd at bignews.shef.ac.uk>, G Garcia-asua
<MBP95GG at shef.ac.uk> writes
>Rapid analysis of plasmids containing the insert can be done (according 
>to Maniatis) by resuspending the colonies in SDS, heating them and load 
>them into an agarose gel without ethidium bromide. After 
>electrophoresis the staining with EtBr should be done to visualise the 
>DNA and select the recombinants. 
>
>My question is: does this actually work even with small inserts (i.e. 
>300bp)?
>

Alternatively do PCR with primers flanking your region of interest with
pUC18 use universal + reverse primers.

Duncan
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Dr. Duncan Clark
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