5' overhang on PCR primers

Thomas F. Vogt tvogt at WATSON.PRINCETON.EDU
Tue Jan 28 12:42:05 EST 1997


I want to do PCR with a primer that has a 5'overhang of 
50bp of unpaired nucleotides for the creation of an 
epitope tag. CAN this be done?  Is there a maximum number 
of nucleotides that can be left unpaired and PCR still 
work well.



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