The life time of Luria Broth(LB) media with Ampicillin ?

Michael Douglas mpdougla at
Tue Jan 28 16:27:34 EST 1997


We use LB containing ampicillin (50ug/ml).  It works the best when made
fresh but I have used it up to a month old without any problems.  I
would not use anything older than 1 month stored at 4C.  Also, it is
critical that the media be stored in the dark because some antibiotics
are not stable in the light.

Michael Douglas
Research Assistant
SUNY-Environmental Science and Forestry
Environmental and Forest Biology
Illick Hall
1 Forestry Drive
Syracuse, NY 13210

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