The life time of Luria Broth(LB) media with Ampicillin ?

Lee Daesang dslee at DONALD.HANHYO.CO.KR
Tue Jan 28 08:11:46 EST 1997


Dear netter,

I wonder the life time of Luria Broth( LB ) containg ampicillin (50ug/ml).

Two months age, i made the LB-ampicillin stocks and have stored it in 

cold room ( about 4 ¡£C). At that time it worked good in expressing the 

foreign protein in E. coli as inclusion body, but noedays it doesn't.

I also wonder about the life time of the Ampicillin powder at 4 ¡£C.

If you have any idea about those things, please  dont't hesite to

 write a reply mail.

Thanks in advance.






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