TBE vs. TAE
paquetty at ERE.UMontreal.CA
Tue Jan 28 09:56:15 EST 1997
ladasky at leland.Stanford.EDU (John Ladasky) writes:
> I'm starting to wonder whether TBE is worth it. Every two months
>or so, I have to make a new 10X stock because it has precipitated. And
>when I do band purifications from agarose gels, I have to add more sodium
>perchlorate than if I use TAE, with a concomitant loss in DNA yield. Mean-
>while, I have a 50X stock solution of TAE that I made up a year ago which
>I still use.
> The only thin g that I can find in the literature is that TBE will
>give better resolution of high molecular-weight bands on an agarose gel
>(10 kB - 30 kB). Is there any other justification for the use of TBE be-
>sides this? And could one run sequencing gels with TAE instead of TBE?
>Unique ID : Ladasky, John Joseph Jr.
>Title : BA Biochemistry, U.C. Berkeley, 1989 (Ph.D. perhaps 1998???)
>Location : Stanford University, Dept. of Structural Biology, Fairchild D-105
>Keywords : immunology, music, running, Green
One advantage of TBE over TAE is that it can be run at a higher voltage because it carries less current.
However, you should try TTE (Tris 89 mM, taurine 28.5mM and EDTA 0.5mM for a 1X
solution). This buffer works well with agarose gels and sequencing gels and does
not interfere with DNA isolation from agarose. I usually prepare a 20X solution
(Tris 1.78M, taurine 0.57M and EDTA 10mM). This stock solution does not form a
precipitate and is stable for several months. I have used this buffer in my lab
for two years now for every gel that i run, and I would not change back to TAE or TBE.
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