5' overhang on PCR primers

Jillian Johnston johnstoj at acs2.acs.ucalgary.ca
Tue Jan 28 15:41:22 EST 1997


Once you get past the first few rounds of PCR you will probably
be OK because there will a template for the whole primer.


Thomas F. Vogt (tvogt at WATSON.PRINCETON.EDU) wrote:
: I want to do PCR with a primer that has a 5'overhang of 
: 50bp of unpaired nucleotides for the creation of an 
: epitope tag. CAN this be done?  Is there a maximum number 
: of nucleotides that can be left unpaired and PCR still 
: work well.

--
Jillian Johnston
Dept. of Biological Sciences       Guider
Univ. of Calgary         	   30 Pathfinders, Tanisi District




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