5' overhang on PCR primers
johnstoj at acs2.acs.ucalgary.ca
Tue Jan 28 15:41:22 EST 1997
Once you get past the first few rounds of PCR you will probably
be OK because there will a template for the whole primer.
Thomas F. Vogt (tvogt at WATSON.PRINCETON.EDU) wrote:
: I want to do PCR with a primer that has a 5'overhang of
: 50bp of unpaired nucleotides for the creation of an
: epitope tag. CAN this be done? Is there a maximum number
: of nucleotides that can be left unpaired and PCR still
: work well.
Dept. of Biological Sciences Guider
Univ. of Calgary 30 Pathfinders, Tanisi District
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