painful gel loading???!!!!!!

xudong huang xudong.huang at
Wed Jan 29 11:42:08 EST 1997


I have a painful problem about agarose gel loading. The DNA samples do
not stay at the bottom of wells, intead, they float out of wells and
diffuse into the buffer. The loading buffer I use is 6XBPB, the gel
running buffer is 1X TBE. It is not because of air bubbles or oil in the
samples. Could you let me know what is going on? Thank you.

Xudong Huang
email:xudong.huang at
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