painful gel loading???!!!!!!

Elizabeth Rogers erogers at
Wed Jan 29 15:20:30 EST 1997

On Wed, 29 Jan 1997, xudong huang wrote:

> Hello,
> I have a painful problem about agarose gel loading. The DNA samples do
> not stay at the bottom of wells, intead, they float out of wells and
> diffuse into the buffer. The loading buffer I use is 6XBPB, the gel
> running buffer is 1X TBE. It is not because of air bubbles or oil in the
> samples. Could you let me know what is going on? Thank you.
> Xudong Huang
> email:xudong.huang at
 I've had this problem when I've had SDS in my sample.   Beth

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