PCR specificity

Ed Wang ed_wang at arris.com
Wed Jan 29 11:20:15 EST 1997


Yes...Mg++ shields the repulsion createtd by the negative charges
between the template and the primers (which is part of the force
contributing to the specificity of the primers).  To an extent, the more
Mg++, the more locations along the template a primer will bind. 
Therefore, by increasing the Mg concentration, you are allowing the
primers to bind at more than one place, creating multiple PCR products.





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