Aliquoting Antibodies for freezing - HELP!
gjenkins at facstaff.wisc.edu
Wed Jan 29 14:24:46 EST 1997
I have always made aliquots and flash froze them in dry ice/ETOH.
If they have been affinity purified I add BSA to 1%
Never had a problem.
In article <01bc0df0$1e5db0e0$6a0b6883 at general.uoguelph.ca>, "Jen
Robertson" <jvrobert at uoguelph.ca> wrote:
> I hope someone can give me advice on how to aliquot polyclonal IgG. I've
> been told to use 100% glycerin with added sodium azide. BUT the problem is
> that the glycerin is so gummy by the time the whole amount gets sucked into
> the pipette (250ul). I need help on this!!!! Any other suggestions. Please
> Jen :)
> jvrobert at uoguelph.ca
Glenn H. Jenkins
Dept of Human Oncology
University of Wisconsin
Madison, WI 53792
Ph (608) 263-4084
Fx (608) 263-4226
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