Vol of ligation mix to use in transformation
seemysignature at mole.bio.cam.ac.-uk
Thu Jan 30 07:23:01 EST 1997
In article <32F05C68.4369 at sheffield.ac.uk>, K.Mulcahy at sheffield.ac.uk wrote:
>Also, does anyone know the answer to the following question? When adding
>your ligation mix to the bacteria (without precipitation of the DNA
>first) why is it recommended (e.g. in the Gibco T4 DNA ligase protocol)
>that you dilute the ligation mix more than 5 times before adding it to
There was a BRL Focus article (ie Gibco!) on optimizing ligation
conditions. They showed that diluting the ligation mix results in a much
higher transformation efficiency. (Presumably there is some inhibitory
component in ligation mix).
I can probably dig out the exact reference if you want it.
Wellcome/CRC Institute, Time flies like an arrow...
Tennis Court Road,
Cambridge CB2 1QR Fruit flies like a banana.
Tel: [+44] (0)1223 334129 Email:drm21 at mole.bio.cam.ac.-uk
Fax: [+44] (0)1223 334089
** To avoid unsolicited Email I have doctored my return address**
**Please use the above, with the "-" sign edited out. **
More information about the Methods