painful gel loading???!!!!!!

Darren Natale dnatale at box-d.nih.gov
Thu Jan 30 13:20:16 EST 1997


Mike Irwin wrote:
> 
> In article <32F0A569.7F47 at box-d.nih.gov>, Darren Natale
> <dnatale at box-d.nih.gov> wrote:
> 
> > samples were PCR reactions (performed without mineral oil).  I loaded
> > half the
> > reaction onto the gel, only to have them float out in streams of blue.
> > After a
> > fresh buffer change, I loaded the remaining half without incident.
> 
> In that case, we're probably talking about contamination in your loading
> buffer where your bugs ate the glycerol or other "weighting" substance.

Nope.  The loading buffer was added to the PCR reaction directly, BEFORE 
the first gel.  The ONLY difference between the two gels was the buffer.
In fact, even the gel was the same (I simply used the remaining lanes).

D. Natale



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