natalieB at qimr.edu.au
Thu Jan 30 23:17:59 EST 1997
ags at MOLE.BIO.CAM.AC.UK wrote:
: i am working with Staphylococcus aureus and i would like to put two questions
: to the group with the hope someone has an answer:
: 1. If you are using lysostaphin, is there any source for obtaining it you
: recommend, cheaper for example. I've heard of a supplier, a USA company
: which has a recombinant form which is cheaper than the Sigma one.
: 2. Is there any reliable method of obtaining plasmids from S.aureus, i.e.
: Thanks in advance
I did some plasmid extraction from Staph using a pulse field electrophoresis. After running the gel, you could use beta agarase to purify the plasmid. It should work. Good luck. NatalieZZDI
did d: ags at mole.bio.cam.ac.uk
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