The life time of Luria Broth(LB) media with Ampicillin ?

Steven Cohen sbc4 at PO.CWRU.EDU
Fri Jan 31 17:01:52 EST 1997


I agree that it is best to add the ampicillin directly to the LB just before
using from an Amp Stock.

But to avoid mutiple freeze thaws, (even of your small alaquots), dissolve
Amp at 1000X in 70% ETOH. This will not solidify at  -20, and therefore
never needs to be thawed/melted.




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