large amount of mRNA is needed
shelley at DARWIN.SFBR.ORG
Fri Jan 31 14:02:56 EST 1997
Ambion sells some kits that use some sort of modified polymerase or
buffer that will in vitro transcribe large amounts (mgs) of message. I
have used their MEGAShortscript kit with success, but do not know their
secret. I believe their kits work with T7 or Sp6 promoters for any
I hope this helps answer your question?
On 31 Jan 1997, Wei Shao wrote:
> Hello all, is there any vector good for making large amount of mRNA
> from a cloned gene? I do not need expressed protein, only need
> mRNA. Promega has some trancription vectors. I think those are for
> isolating mRNA for in vitro transcription. What I need is isolating
> mRNA of cloned gene from bacterial in mg level-- Is it possible?
More information about the Methods