blue-harts

Edwin Abeln e.c.a.abeln at biol.rug.nl
Tue Jul 1 02:35:19 EST 1997


I did a pBluescript transformation in XL1 blue with inserts between
300 and 800 basepair and got white and blue colonies. However many 
of the whites have a blue to blue-ish center. Also my pGEMT transformation 
had the same problem.Does anyone have an explanation for this? 
(I though myself of small in frame inserts.
Instability of X-gal seems a more remote possibility.)

Edwin Abeln




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