Disposable Columns

Jared Head bijgh at zeus.bris.ac.uk
Tue Jul 1 05:07:15 EST 1997


Hi,

I've been making home-made affinity columns by putting some glass wool at
the bottom of a disposable 20ml syringe and pouring the sepharose in on
top.  It works, but is a bit unreliable; sometimes the glass wool allows
resin to leak out, other times there's too much glass wool and the drip
rate is very slow.

Has anybody got any better methods for keeping the resin in, or can you
buy cheap plastic columns with a filter already in the bottom (which would
be ideal)?

Thanks in advance,

Jared

-- 
Jared Head     at the Department of Biochemistry, University of Bristol

"A computer lets you make more mistakes faster than any invention in human
     history - with the possible exceptions of handguns and tequila." 
                                                   Mitch Ratliffe



More information about the Methods mailing list