Protein for DNAse footprinting

JOSE LUIS CARRASCO JIMENEZ jlcarrasco at ibmcp.upv.es
Tue Jul 1 13:10:59 EST 1997


  Dear colleagues,

  I am interested in doing in vitro DNAse footprinting
assays, and I wonder whether it might be possible to use
whole cell extracts as a protein source instead of nuclear
extracts, since I have detected clear, specific binding by 
gel retardation assay using such extracts.

  Does anybody experience in this respect? Any opinion
will be welcome.

  Thanks in advance.

  Jose L. Carrasco
  jlcarrasco at ibmcp.upv.es



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