P1 nuclease

beyers at uctvms.uct.ac.za beyers at uctvms.uct.ac.za
Wed Jul 2 09:02:47 EST 1997


I've been trying to use P1 nuclease to nibble back a 5'
overhang and thereby create a blunt end. I use Boehringer buffer
H for the reaction, but so far I haven't had any success. Can
anyone please give me some advice!

Thanks in advance
Gordon Brown

Please reply to Gbro at gerga.sun.ac.za

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