Frank O. Fackelmayer
fof1 at chclu.chemie.uni-konstanz.de
Wed Jul 2 05:39:50 EST 1997
In article <ECMxG3.LDz at fsa.bris.ac.uk>, bijgh at zeus.bris.ac.uk (Jared Head)
> I've been making home-made affinity columns by putting some glass wool at
> the bottom of a disposable 20ml syringe and pouring the sepharose in on
> top. It works, but is a bit unreliable; sometimes the glass wool allows
> resin to leak out, other times there's too much glass wool and the drip
> rate is very slow.
> Has anybody got any better methods for keeping the resin in, or can you
> buy cheap plastic columns with a filter already in the bottom (which would
> be ideal)?
> Thanks in advance,
> Jared Head at the Department of Biochemistry, University of Bristol
> "A computer lets you make more mistakes faster than any invention in human
> history - with the possible exceptions of handguns and tequila."
> Mitch Ratliffe
We routinely use the disposable plastic columns from Biorad (no
affilitation), they appear to be exactly what you need.
Hope this helps,
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