Disposable Columns

Frank O. Fackelmayer fof1 at chclu.chemie.uni-konstanz.de
Wed Jul 2 05:39:50 EST 1997


In article <ECMxG3.LDz at fsa.bris.ac.uk>, bijgh at zeus.bris.ac.uk (Jared Head)
wrote:

> Hi,
> 
> I've been making home-made affinity columns by putting some glass wool at
> the bottom of a disposable 20ml syringe and pouring the sepharose in on
> top.  It works, but is a bit unreliable; sometimes the glass wool allows
> resin to leak out, other times there's too much glass wool and the drip
> rate is very slow.
> 
> Has anybody got any better methods for keeping the resin in, or can you
> buy cheap plastic columns with a filter already in the bottom (which would
> be ideal)?
> 
> Thanks in advance,
> 
> Jared
> 
> -- 
> Jared Head     at the Department of Biochemistry, University of Bristol
> 
> "A computer lets you make more mistakes faster than any invention in human
>      history - with the possible exceptions of handguns and tequila." 
>                                                    Mitch Ratliffe


Hi Jared,
We routinely use the disposable plastic columns from Biorad (no
affilitation), they appear to be exactly what you need.

Hope this helps,
Frank



More information about the Methods mailing list