DNase footprinting/gel shift

JOSE LUIS CARRASCO JIMENEZ jlcarrasco at ibmcp.upv.es
Wed Jul 2 06:08:28 EST 1997


  Dear colleagues,

  I would be grateful if someone could provide
me with a complete protocol for DNase footprinting 
in combination with gel retardation assay. I have 
heard that it is easier to incubate the binding 
mixtures with DNase I prior to running the retardation 
gel, and then elute the nicked DNA from the retarded
complex. How is this done? Any other alternative
protocol will also be appreciated.

  Thanks alot.

  Jose L. Carrasco
  jlcarrasco at ibmcp.upv.es



More information about the Methods mailing list