P1 nuclease

brett brett at BORCIM.WUSTL.EDU
Wed Jul 2 09:58:56 EST 1997


>Hi 
>
>I've been trying to use P1 nuclease to nibble back a 5'
>overhang and thereby create a blunt end. I use Boehringer buffer
>H for the reaction, but so far I haven't had any success. Can
>anyone please give me some advice!
>
>Thanks in advance
>Gordon Brown
>
>Please reply to Gbro at gerga.sun.ac.za
>
>
>

mung bean




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