Help!!! difficulties in dephosphorylating a protein

Anguenot Raphaël aai395 at agora.ulaval.ca
Wed Jul 2 11:01:32 EST 1997


Hello Friends,
> We are working with a plant protein that is totally phosphorylated in
> vivo.  Assays of dephosphorylation using alkaline phosphatase (CIP) or
> acid potato phosphatase were inefficient.  We have used different
> conditions, but removal of 32-P was not higher than 20% (2 hours
> incubation).  Does anyone encounter such problem with non-specific
> phosphatases? Is there anyone who knows a protocol using general
> phosphatases which works properly ? Any lab protocol or references are
> welcomed.
>
> Kindly reply to me or the respective group.
>
> Thanks in advance
>
> Raphael
>
> Email:
> aai395 at agora.ulaval.ca



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