Need protocol for lacZ staining
pnorton at lac.jci.tju.edu
Thu Jul 3 13:13:29 EST 1997
In article <33B7CC5F.507C at glaxowellcome.com>, Gary Kucera
<kucera~gt at glaxowellcome.com> wrote:
> Can anyone send me via e-mail a protocol for lacZ staining of mouse
> embryos (or culutured eukaryotic cells). The protocol I have is giving
> me a headache.
> Gary T. Kucera, Research Scientist
> Functional Genetics/Transgenics Group
> GlaxoWellcome Research, Inc.
> Five Moore Drive
> Research Triangle Park, NC 27709
> kucera~gt at glaxowellcome.com
I highly recommend that you check out the following URL:
In addition to lots of great info on mice and rats, there is a link to
a long guide to lacZ staining, with many hints and tips. Thanks to Eric
Mercer for creating this resource.
I can send a copy of our protocol to you. However, it might be helpful
if you provide a little more detail one just why your protocol is giving
you a headache. Background? Too light? etc...
Pamela A. Norton, Ph.D. Associate Professor of Medicine
Thomas Jefferson University
Philadelphia, PA 19107 p_norton at lac.jci.tju.edu
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