PCR screening of bacterial colonies

John Watson watson_j at bms.com
Thu Jul 3 15:18:56 EST 1997

Minsoo Yoon wrote:
> In my experience, there has been no need for any other manipulations (such
> as boiling) other than just touching the colonies with sterile toothpicks
> and then swirling it directly in the PCR reaction mix. I always get clear
> results with that.

I have to agree w/ Minsoo Yoon.  We don't manipulate the the colonies
either.  Just touch, swirl and amplify.

John Watson
Bristol-Myers Squibb Co.
watson_j at bms.com
"If you're not part of the solution, you're part of the precipitate."

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