SYPRO orange stain on PhastSystem gels ?
vkraynov at pop.service.ohio-state.edu
Thu Jul 3 23:10:53 EST 1997
Thanks for this suggestion ! You are probably right - I 'm not sure what the
gel backing consists of, but it probably does block UV. I was thinking about
removing the backing somehow, but the gel is really fragile, and I couldn't
find the way. I'll try illuminating it from above.
The problem , however, is that I NEED to TRANSilluminate in order to
This dye, if works, would provide a great alternative to time-consuming
silver staining, with practically the same sensitivity, plus easy
"photographability" - just like regular EB stained agarose DNA gels. Cheers
In article <33BC5F95.41C6 at gandalf.psf.sickkids.on.ca>, Randy Willis
<willis at gandalf.psf.sickkids.on.ca> wrote:
>Vadim Kraynov wrote:
>> Did anybody successfully stain Pharmacia's Phast System protein SDS gels
>> (with plastic backing) with BioRad's SYPRO orange protein stain ?
>> I want to make a photo of my gel, and can't seem to be able to stain it with
>> this new stain, which supposed to fluoresce under UV...
>I don't mean this to sound condescending, but are you sure that the UV
>light transmits through the plastic backing? If possible, try shining a
>UV lamp on the stained gel from above to see if the proteins are
>stained. You may also want to call Pharmacia to see if they have any
>ideas about the backing and UV light. If you do, please be sure to post
>the result on the newsgroup.
>Best of luck,
>Randall C Willis, Researcher
>Biochemistry Research, Hospital for Sick Children
>3522-555 University Ave
>M5G 1X8 CANADA
>416-813-5933 (ph) -5022 (fax)
>willis at gandalf.psf.sickkids.on.ca
More information about the Methods