BEST METHOD TO FIX RNA IN NYLON MEMBRANE
diego at DNA.CBIOT.UFRGS.BR
Thu Jul 3 16:04:37 EST 1997
I want to fix total RNA of S. cerevisiae on NyLon membrane
(Hybond-N, uncharged, Amersham). What is the best method for it
(heat at 80 Celsius degrees for 2 hours or UV crosslink)? I don't
have commercial UV crosslinker.
I like to know too if in both case can I reutilize the membrane for
Any suggest is welcome.
diego at dna.cbiot.ufrgs.br
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