BEST METHOD TO FIX RNA IN NYLON MEMBRANE

Diego Bonatto diego at DNA.CBIOT.UFRGS.BR
Thu Jul 3 16:04:37 EST 1997


Hello:

   I want to fix total RNA of S. cerevisiae on NyLon membrane 
(Hybond-N, uncharged, Amersham).  What is the best method for it 
(heat at 80 Celsius degrees for 2 hours or UV crosslink)? I don't 
have commercial UV crosslinker. 
   I like to know too if in both case can I reutilize the membrane for 
new hibridizations.
  Any suggest is welcome.
  Thanks
  Diego Bonatto
  diego at dna.cbiot.ufrgs.br
   



More information about the Methods mailing list