gschlue at gwdg.de gschlue at gwdg.de
Fri Jul 4 04:02:45 EST 1997


I try to establish regulatable transgenic lines, using the TetON/OFF
system but it seems that the VP16 domain in the transactivator is causing
trouble, at least with strong promotors (e.g. PGK driven expression
of rtTA seems to be rather deadly).Does anybody know which is the
essential core of this VP16 domain or did anybody create a modified
transactivation domain which works well ????

Desperately seeking

Gregor Schlueter

-------------------==== Posted via Deja News ====-----------------------
      http://www.dejanews.com/     Search, Read, Post to Usenet

More information about the Methods mailing list