beta-gal in Jurkat cells

Eric Anderson e-anderson at ski.mskcc.org
Fri Jul 4 12:19:21 EST 1997


In article <33BB852B.3669 at uci.edu>, mqn1 at uci.edu wrote:

>Hi,
>
>Have anyone try measuring beta-gal activity in Jurkat cells?  We try but
>without success.  The beta-gal is in pCMV5 (which works in Cos cells). 
>We tried different lysing methods, but that didn't work either.  Any
>suggestions?  Thanks.
>
>mqn

we do it all the time in this lab.  3-5 cycles of freeze/thaw (37oC -->
dry ice/EtOH bath, about 4-5 min. each) does the trick almost every time.

eric

-- 
Eric C. Anderson
Sloan-Kettering Institute for Cancer Research 
Dept. of Cell Biology and Genetics
Lab: (212) 639-2977
Fax: (212) 717-3298
e-anderson at ski.mskcc.org



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