Any experiences with pET 31b(+) Vector and KSI-Fragment purification?
leif.dehmelt at mpi-dortmund.mpg.de
Fri Jul 4 06:06:53 EST 1997
I would like to read about some good or bad experiences
with the pET 31b(+) Vector from NOVAGEN. This Vector decodes
for a KSI-Fragment Fusion with C-terminal His-Tag. The KSI
(Ketosteroid-Isomerase)-Fragment leads to insolubility and strong
overexpression in inclusion bodies, even for small proteins or peptides
in fusion (17-65 as). The KSI-Fusion is cleaved by CNBr and the following
seperation step is achieved by simple centrifugation.
Does anybody have particular practical experiences with this system?
Thanks for every reply...
More information about the Methods