High Percentage SDS-PAGE gels

[Troels Wind]

I've made 18% gels without problems, any higher?

Troels

Tim Fitzmaurice (tjf11 at cus.cam.ac.uk) wrote:

: JUst a small plea for help before I try something. Is anyone out there
: aware of any problems/tricks involved in getting good polyacrylamide gels
: of higher than 15% or is it just mix up the higher amount of acrylamide 
: and go.

: Oh, and this is in minigels for protein rather than sequencing gels.

: Thanks,
: Tim