Cleavage by thrombin

Simon Hoffenberg simonh at bcm.tmc.edu
Sun Jul 6 19:45:42 EST 1997


Hi netters,

Did anybody have negative experience with thrombin, when thrombin digests
the protein cleaved from GST? We used high thrombin concentration to
accelerate the digestion and lost our protein. Any cure for the problem?

Simon Hoffenberg
Baylor College of Medicine
Houston, TX
simonh at bcm.tmc.edu



More information about the Methods mailing list