Cleavage by thrombin

Simon Hoffenberg simonh at
Sun Jul 6 19:45:42 EST 1997

Hi netters,

Did anybody have negative experience with thrombin, when thrombin digests
the protein cleaved from GST? We used high thrombin concentration to
accelerate the digestion and lost our protein. Any cure for the problem?

Simon Hoffenberg
Baylor College of Medicine
Houston, TX
simonh at

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