Single-stranded nicks in plasmids

Kevin Williamson ksw at novadx.com
Mon Jul 7 19:02:23 EST 1997


I need to know how to introduce a nick into one strand of a ds-plasmid 
so that I can use ExonucleaseIII to remove the nicked strand- 
effectively converting a double-stranded plasmid library into a single 
stranded one for hybridisation.
Please reply to proben at ucsd.edu

Thanks!



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