HELP!!! DNAse treating human adipose RNA

Ivor Percent percenti at MHS.PBRC.EDU
Tue Jul 8 15:14:42 EST 1997


I RNA extracted from human adipose and then DNAse treated then heat
deactivate it. Every time I DNAse treat my sample it is degraded. I have
run all possible controls i.e. buffer and sample only DNASE + sample
only ECT.... I still get degraded samples. I ran the same DNAse and
buffer on a rat RNA and the samples were fine. I am useing RNAse free
DNAse from pharmacia . Any sugestions, please help!! , contact me at
percenti at mhs.pbrc.edu 
thanks 
ivor percent 


                                                                        
                                                                        
                                                                        
                                                                        
                                                                        
                                                                        
                                                                        
                                                                        
                                                                        
                                                                        
                                                               



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