HELP! -- Protecting DNA on a transilluminator?

Alexander N. Kukushkin kan at mmcd.cyt.ras.spb.ru
Tue Jul 8 11:13:21 EST 1997


>   I am having problems ligating my DNA fragments cut out of a gel.  I
>   get variable results that seem to relate  to how long it takes to cut
>   out the band.  The fastest I can see and cut out a band is about 15
>   seconds and by then I suspect there is already significant UV damage.
>   Is there any way to protect my DNA during UV exposure?
>
In last year there was a report in BioTechniques (21, 898) about DNA
protection in the agarose gel against UV damage by the addition of
cytidine or guanosine to the electrophoresis buffer.
Alex



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