Stu I problems

Mozart de Azevedo Marins mdamarin at usp.br
Tue Jul 8 08:58:30 EST 1997


Hi there,

          I have a problem with a Stu I digestion. I have an insert which 
contains a Stu I site that in the 5' end is overllaping with a metilation 
site...it forms ccAGGCCT...the ccAGG is the metilaiton site and the 
AGGCCT the Stu I site. The C to be metilated would be the first one, out 
of the Stu I site, and I don't know if this really interferes with the 
restriction. Bfeore i change to a dcm starin I would like to know if I am 
missing something...




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