blue-harts

Feodor A. Brovko brovko at fibkh.serpukhov.su
Tue Jul 8 08:02:45 EST 1997


Dear Edwin !
Let your plates cool at +4 during 2-3 days. Then your true-white colonies
retain white and others became blue. 

Edwin Abeln <e.c.a.abeln at biol.rug.nl> ÚÁÐÉÓÁÎÏ × ÓÔÁÔØÀ
<5pabvn$see$1 at info.service.rug.nl>...
> I did a pBluescript transformation in XL1 blue with inserts between
> 300 and 800 basepair and got white and blue colonies. However many 
> of the whites have a blue to blue-ish center. Also my pGEMT
transformation 
> had the same problem.Does anyone have an explanation for this? 
> (I though myself of small in frame inserts.
> Instability of X-gal seems a more remote possibility.)
> 
> Edwin Abeln
> 
> 



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