Feodor A. Brovko
brovko at fibkh.serpukhov.su
Tue Jul 8 08:02:45 EST 1997
Dear Edwin !
Let your plates cool at +4 during 2-3 days. Then your true-white colonies
retain white and others became blue.
Edwin Abeln <e.c.a.abeln at biol.rug.nl> ÚÁÐÉÓÁÎÏ × ÓÔÁÔØÀ
<5pabvn$see$1 at info.service.rug.nl>...
> I did a pBluescript transformation in XL1 blue with inserts between
> 300 and 800 basepair and got white and blue colonies. However many
> of the whites have a blue to blue-ish center. Also my pGEMT
> had the same problem.Does anyone have an explanation for this?
> (I though myself of small in frame inserts.
> Instability of X-gal seems a more remote possibility.)
> Edwin Abeln
More information about the Methods